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rabbit polyclonal antibodies anti cdk2  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit polyclonal antibodies anti cdk2
    Rabbit Polyclonal Antibodies Anti Cdk2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 254 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibodies+anti+cdk2/Phospho-CDK2+(Thr160)+Antibody/pmc12143771-256-10-15
    Average 95 stars, based on 254 article reviews
    rabbit polyclonal antibodies anti cdk2 - by Bioz Stars, 2026-10
    95/100 stars

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    Incubation:

    Article Title: KRAS Inhibition Reverses Chemotherapy Resistance Promoted by Therapy-Induced Senescence-like in Pancreatic Ductal Adenocarcinoma
    Article Snippet: .. Next, they were incubated overnight at 4°C with the rabbit polyclonal antibodies anti-CDK2 (1:1,000; Cell Signaling Technology), anti-CDK4 (1:2,000; Cell Signaling Technology), anti-CDK6 (1:1,000; Cell Signaling Technology), anti-Ki67 (1:1,000; Cell Signaling Technology), anti-p21 (1:1,000; Cell Signaling Technology), anti-p27 (1:1,000; Cell Signaling Technology), anti-ZEB1 (1:1,000; Cell Signaling Technology), anti-Vimentin (1:1,000; Cell Signaling Technology), anti-ERK (1:1,000; Cell Signaling Technology), anti-pERK (1:1,000; Cell Signaling Technology), anti-AKT(1:1,000; Cell Signaling Technology) anti-pAKT S473 (1:1,000; Cell Signaling Technology), anti-pPDK1 S241 (1:1,000; Cell Signaling Technology), anti-pAKT S308 (1:1,000; Cell Signaling Technology), anti-p-PTEN S380 (1:1,000; Cell Signaling Technology), For the immunoreaction, the membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:3,000 dilution, Suther Biotech) or HRP-conjugated rabbit anti-goat IgG (1:3,000 dilution, Suther Biotech). .. The outcome was visualized using the Chemiluminescent HRP substrates (Millipore Corporation) for chemiluminescence development.

    Article Title: KRAS inhibition reverses chemotherapy resistance promoted by therapy-induced senescence-like in pancreatic ductal adenocarcinoma
    Article Snippet: Proteins were electro-transferred to a nitrocellulose membrane (Immobilon-P, EMD Millipore Corporation) at 250 mA for 2 h. To identify proteins, the membranes were blocked for 1 hour at room temperature with 5 % powdered milk in PBS containing 0.1 % Tween 20. .. Next, they were incubated overnight at 4 °C with the rabbit polyclonal antibodies anti-CDK2 (1:1000; Cell Signaling Technology), anti-CDK4 (1:2000; Cell Signaling Technology), anti-CDK6 (1:1000; Cell Signaling Technology), anti-Ki67 (1:1000; Cell Signaling Technology), anti-p21 (1:1000; Cell Signaling Technology), anti-p27 (1:1000; Cell Signaling Technology), anti-ZEB1 (1:1000; Cell Signaling Technology), anti-Vimentin (1:1000; Cell Signaling Technology), anti- ERK (1:1000; Cell Signaling Technology), anti-pERK (1:1000; Cell Signaling Technology), anti-AKT(1:1000; Cell Signaling Technology) anti-pAKT S473 (1:1000; Cell Signaling Technology), anti-pPDK1 S241 (1:1000; Cell Signaling Technology), anti-pAKT S308 (1:1000; Cell Signaling Technology), anti-p-PTEN S380 (1:1000; Cell Signaling Technology), For the immunoreaction, the membranes were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:3000 dilution, Suther Biotech) or HRP-conjugated rabbit anti-goat IgG (1:3000 dilution, Suther Biotech). .. The outcome was visualized using the Chemiluminescent HRP substrates (Millipore Corporation) for chemiluminescence development.



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    Image Search Results


    Journal: Cell reports

    Article Title: Hepatic Snai1 and Snai2 promote liver regeneration and suppress liver fibrosis in mice

    doi: 10.1016/j.celrep.2024.113875

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-CDK2 , Proteintech Group , Cat#10122–1-AP; RRID:AB_2078556.

    Techniques: Plasmid Preparation, Virus, In Situ, Real-time Polymerase Chain Reaction, Recombinant, Luciferase, Software

    ZQD caused NSCLC apoptosis and cell cycle arrest in vivo experiments. ( A ) Apoptotic rates of cancer cells based on TUNEL assay. The image is representative of three animals per experimental group (magnification × 200). ( B, C ) Bax, Bcl2, and CDK2 relative expression in the tumor was determined by Western blot; ZQD-L/H significantly increased Bax expression while decreasing Bcl2 and CDK2 expression in NSCLC. ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01. Data represented as the mean (±SEM) of at least three independent experiments, each performed in triplicate.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Ze-Qi decoction inhibits non-small cell lung cancer growth and metastasis by modulating the PI3K/Akt/p53 signaling pathway

    doi: 10.1016/j.jtcme.2023.03.008

    Figure Lengend Snippet: ZQD caused NSCLC apoptosis and cell cycle arrest in vivo experiments. ( A ) Apoptotic rates of cancer cells based on TUNEL assay. The image is representative of three animals per experimental group (magnification × 200). ( B, C ) Bax, Bcl2, and CDK2 relative expression in the tumor was determined by Western blot; ZQD-L/H significantly increased Bax expression while decreasing Bcl2 and CDK2 expression in NSCLC. ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01. Data represented as the mean (±SEM) of at least three independent experiments, each performed in triplicate.

    Article Snippet: The antibodies used were rabbit anti-matrix metalloproteinase 2 (anti-MMP2; 1:1000, 10373-2-AP; Proteintech, Wuhan, China), rabbit anti-MMP9 (1:1000, 10375-2-AP; Proteintech, Wuhan, China), rabbit anti-CD31 (1:1000, ER31219; Huabio, Hangzhou, China), rabbit anti-vascular endothelial growth factor A (anti-VEGFA; 1:1000, ET1604-28; Huabio, Hangzhou, China), mouse anti-P53 (1:1000, 60,283-2-lg; Proteintech, Wuhan, China), rabbit anti-cyclin-dependent kinase 2 (anti-CDK2; 1:1000, bs-10726R; Bioss, Beijing, China), mouse anti-phosphoinositide 3-kinase (anti-PI3K; 1:3000, 67071-1-Ig; Proteintech, Wuhan, China), rabbit anti- P -PI3K (1:1000, bs-5570R; Bioss, Beijing, China), mouse anti-protein kinase B (anti-AKT; 1:20000, 60203-2-Ig; Proteintech, Wuhan, China), mouse anti- P -AKT (1:5000, 66444-1-Ig; Proteintech, Wuhan, China), rabbit anti-Bcl2 associated X-protein (anti-Bax; 1:5000, ab32503; Abcam, Cambridge, UK), rabbit anti-B-cell lymphoma 2 (anti-Bcl2; 1:2000, ab32503; Abcam, Cambridge, UK), and rabbit anti-HRP-conjugated β-actin (1:20,000, HRP-60008; Proteintech, Wuhan, China).

    Techniques: In Vivo, TUNEL Assay, Expressing, Western Blot