rabbit polyclonal antibodies anti cdk2  (Cell Signaling Technology Inc)

Journal: Cell reports

Article Title: Hepatic Snai1 and Snai2 promote liver regeneration and suppress liver fibrosis in mice

doi: 10.1016/j.celrep.2024.113875

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-CDK2 , Proteintech Group , Cat#10122–1-AP; RRID:AB_2078556.

Techniques: Plasmid Preparation, Virus, In Situ, Real-time Polymerase Chain Reaction, Recombinant, Luciferase, Software

Journal: Journal of Traditional and Complementary Medicine

Article Title: Ze-Qi decoction inhibits non-small cell lung cancer growth and metastasis by modulating the PI3K/Akt/p53 signaling pathway

doi: 10.1016/j.jtcme.2023.03.008

Figure Lengend Snippet: ZQD caused NSCLC apoptosis and cell cycle arrest in vivo experiments. ( A ) Apoptotic rates of cancer cells based on TUNEL assay. The image is representative of three animals per experimental group (magnification × 200). ( B, C ) Bax, Bcl2, and CDK2 relative expression in the tumor was determined by Western blot; ZQD-L/H significantly increased Bax expression while decreasing Bcl2 and CDK2 expression in NSCLC. ns, no significance, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01. Data represented as the mean (±SEM) of at least three independent experiments, each performed in triplicate.

Article Snippet: The antibodies used were rabbit anti-matrix metalloproteinase 2 (anti-MMP2; 1:1000, 10373-2-AP; Proteintech, Wuhan, China), rabbit anti-MMP9 (1:1000, 10375-2-AP; Proteintech, Wuhan, China), rabbit anti-CD31 (1:1000, ER31219; Huabio, Hangzhou, China), rabbit anti-vascular endothelial growth factor A (anti-VEGFA; 1:1000, ET1604-28; Huabio, Hangzhou, China), mouse anti-P53 (1:1000, 60,283-2-lg; Proteintech, Wuhan, China), rabbit anti-cyclin-dependent kinase 2 (anti-CDK2; 1:1000, bs-10726R; Bioss, Beijing, China), mouse anti-phosphoinositide 3-kinase (anti-PI3K; 1:3000, 67071-1-Ig; Proteintech, Wuhan, China), rabbit anti- P -PI3K (1:1000, bs-5570R; Bioss, Beijing, China), mouse anti-protein kinase B (anti-AKT; 1:20000, 60203-2-Ig; Proteintech, Wuhan, China), mouse anti- P -AKT (1:5000, 66444-1-Ig; Proteintech, Wuhan, China), rabbit anti-Bcl2 associated X-protein (anti-Bax; 1:5000, ab32503; Abcam, Cambridge, UK), rabbit anti-B-cell lymphoma 2 (anti-Bcl2; 1:2000, ab32503; Abcam, Cambridge, UK), and rabbit anti-HRP-conjugated β-actin (1:20,000, HRP-60008; Proteintech, Wuhan, China).

Techniques: In Vivo, TUNEL Assay, Expressing, Western Blot